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unmethylated gpppg  (New England Biolabs)


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    New England Biolabs unmethylated gpppg
    Unmethylated Gpppg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+cap+analog/G(5)ppp(5)G+RNA+Cap+Struc+Analog/pm42103131-88-26-28
    Average 95 stars, based on 186 article reviews
    unmethylated gpppg - by Bioz Stars, 2026-09
    95/100 stars

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    (A) A-cap / stemloop IRES reporter RNA carrying the negative control “scramble 1” sequence was mixed into a polysome lysate made from HEK293T cells for five minutes on ice and loaded on a sucrose gradient. (B) The sucrose gradient was fractionated and cDNA was prepared from groups of fractions as shown (numbered 1 -6). qRT-PCR was performed to quantify the exogenous “scramble 1” RNA across the fractions, as compared to endogenous Gapdh mRNA. The “scramble 1” transcript migrated into polysomal fractions 4-6, even though it is not translated .

    Journal: bioRxiv

    Article Title: Evaluating the reliability of tools for mRNA annotation and IRES studies

    doi: 10.64898/2026.03.29.707813

    Figure Lengend Snippet: (A) A-cap / stemloop IRES reporter RNA carrying the negative control “scramble 1” sequence was mixed into a polysome lysate made from HEK293T cells for five minutes on ice and loaded on a sucrose gradient. (B) The sucrose gradient was fractionated and cDNA was prepared from groups of fractions as shown (numbered 1 -6). qRT-PCR was performed to quantify the exogenous “scramble 1” RNA across the fractions, as compared to endogenous Gapdh mRNA. The “scramble 1” transcript migrated into polysomal fractions 4-6, even though it is not translated .

    Article Snippet: The ARCA m 7 G-cap analog (New England Biolabs; S1411) was used at a 4:1 ratio to GFP for transcription of T7-nluc constructs, while an A-cap analog (New England Biolabs S1406), was used to generate T7HP-nluc IRES reporter RNAs.

    Techniques: Negative Control, Sequencing, Quantitative RT-PCR